Table 2.
Colloidal properties of ortho ester-based liposomes and lipoplexes.
| Liposome Composition | Liposomes | Lipoplexes (N/P = 5/1) | ||||
|---|---|---|---|---|---|---|
| D (nm) | Poly-dispersity index | ζ-Potential (mV) | D (nm) | Poly-dispersity index | ζ-Potential (mV) | |
| 1/DOPE | 188±2.6 | 0.14 | 58.7±1.4 | 248±1.8 | 0.14 | 58.3±0.6 |
| 2/DOPE | 147±1.9 | 0.24 | 61.0±1.1 | 266±2.8 | 0.13 | 52.4±0.4 |
| 3 | 169±2.7 | 0.07 | 55.6±3.5 | 273±3.3 | 0.27 | 59.4±1.6 |
| 3/DOPE | 181±2.8 | 0.12 | 55.6±3.0 | 343±5.0 | 0.26 | 56.8±1.2 |
| 3/Chol | 171±3.0 | 0.25 | 48.7±1.8 | 378±9.3 | 0.29 | 54.4±2.0 |
| 4/DOPE | 187±3.0 | 0.17 | 50.2±2.0 | 285±12 | 0.22 | 54.2±0.9 |
| 5 | 186±2.1 | 0.14 | 57.6±1.0 | 238±1.5 | 0.10 | 62.6±2.2 |
| 5/DOPE | 167±2.7 | 0.07 | 58.8±2.6 | 262±3.3 | 0.20 | 57.0±1.3 |
| 5/Chol | 170±2.7 | 0.07 | 67.4±2.2 | 265±3.0 | 0.02 | 53.9±1.6 |
| DOTAP | 175±2.1 | 0.13 | 59.8±2.0 | 239±2.7 | 0.18 | 63.5±1.9 |
| DOTAP/DOPE | 163±1.8 | 0.17 | 61.9±1.5 | 241±1.5 | 0.16 | 66.3±1.6 |
| DOTAP/Chol | 172±4.3 | 0.08 | 65.2±2.4 | 288±4.1 | 0.21 | 67.7±2.1 |
a Samples were measured in 100 mM HEPES buffer, pH 7.4. The hydrodynamic diameter (D) and the ζ-potential values are reported as mean ± standard deviation of three measurements.