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. Author manuscript; available in PMC: 2013 Jun 6.
Published in final edited form as: Cell Metab. 2012 Jun 6;15(6):873–884. doi: 10.1016/j.cmet.2012.05.002

Fig. 3. LIRKO livers fail to accumulate nuclear SREBP-1c upon acute re-feeding of a high carbohydrate diet.

Fig. 3

LIRKO mice and their littermate controls (CON) were fasted for 24 hours and then re-fed a high carbohydrate diet for 6–48 hours. Livers were used to prepare cDNA for real-time PCR analysis as well as microsomal and nuclear extracts. (A) SREBP-1c mRNA was measured using real-time PCR. (B) SREBP-1 precursor and nuclear protein levels were measured by immunoblotting microsomal and nuclear extracts, respectively. Calnexin and lamin are shown as loading controls. Each lane represents extracts prepared from equal aliquots of liver from three mice. (C) De novo lipogenesis was measured as the fraction of newly synthesized palmitate present 24 hours after re-feeding. (D) Hepatic triglycerides were measured after six hours of re-feeding. (E–G) Insig mRNA was measured using real time PCR. (H) Insig1 and Insig2 proteins were measured by immunoblotting microsomal extracts. Each lane represents extracts prepared from equal aliquots of liver from three mice. Error bars represent S.E.M.; n=4–8; *p<0.05 versus control mice. See also Figure S3.