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. Author manuscript; available in PMC: 2013 Jun 6.
Published in final edited form as: Cell Metab. 2012 Jun 6;15(6):873–884. doi: 10.1016/j.cmet.2012.05.002

Fig. 4. Mice with knockdown of the insulin receptor fail to accumulate nuclear SREBP-1c upon acute re-feeding of a high carbohydrate diet.

Fig. 4

Two month old mice homozygous for the floxed allele of the insulin receptor were injected with adenovirus encoding GFP or Cre recombinase, and studied two to three weeks later. Mice were fasted for 24 hours and re-fed a high carbohydrate diet for six hours prior to sacrifice. (A) Liver lysates were subjected to immunoblotting with antibodies against the insulin receptor. (B) Body weight was measured at the time of sacrifice. (C) Triglycerides were measured in plasma taken after a four hour fast. (D) Glucose tolerance testing was performed by fasting mice overnight and then injecting with 1 g/kg glucose, intraperitoneally. (E–F, H–I) RT PCR was used to measure hepatic gene expression. (G) SREBP-1 protein was measured in nuclear extracts (prepared from equal amounts of liver taken from at least three mice per group). (J) Plasma and (K) hepatic triglycerides measured in the re-fed state, at the time of sacrifice. Error bars represent S.E.M.; n=3–5, *p<0.05 versus mice injected with adenovirus encoding GFP. See also Figure S4.