Fig. 5.

Induction of the UGT1 locus by DEX in adult hUGT1*1 and hUGT1/Pxr^−/− mice. Adult hUGT1*1 and hUGT1/Pxr^−/− mice were treated with DEX by intraperitoneal injection for 4 consecutive days at 20 mg/kg per dose. Nontreated mice received solvent and were used as controls. Twenty-four hours after the last dose, liver RNA was prepared and RT and Q-PCR analysis of human UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A9, and murine Cyp3a11 gene expression was performed. Fold induction reflects the change in gene expression between solvent-treated and DEX-treated mice.