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. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: ACS Nano. 2012 May 2;6(6):4966–4972. doi: 10.1021/nn300516g

Figure 3. Representative nuclear mapping of DOX in a dynamic cellular environment.

Figure 3

a, OVCAR8 (drug-sensitive) cells treated with free drug and Nanodox at 0, 30 and 60 min. b, Fixed cell images of drug-sensitive treated with free drug and Nanodox and fixed post 1 hr treatment. Cell nuclei are stained in blue and actin stained in green while the drug is in red. Both free drug and Nanodox show nuclear accumulation. Free drug shows hardly any drug accumulation and Nanodox show nuclear accumulation to some extent. d-e, Plot shows nuclear and non-nuclear intensity of DOX with time in the drug-sensitive cancer cells. Exponential uptake pattern is observed with both free drug and Nanodox in drug-sensitive cells. Nanodox shows significantly higher drug uptake than free DOX (p < 0.01). Most importantly nuclear accumulation of DOX is higher compared to non-nuclear regions for Nanodox.