Skip to main content
. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Dev Biol. 2012 May 14;368(1):127–139. doi: 10.1016/j.ydbio.2012.05.002

Figure 6. Reporter analysis of the predicted RGS3 primary TVC regulatory element.

Figure 6

(A) Diagram of RGS3 reporter constructs numbered by their distance from the translation start codon along with the resulting expression levels in the TVCs and other lineages (+++ and + as described in Fig. 3; ++ = ~10–20% of stained embryos; +/−= no expression in some trials but sporadic expression at very low levels in other trials). At the bottom of panel (A) is a graphical representation of nucleosome occupancy scores (Segal et al; 2006) for the predicted CRE taken from the Aniseed Genome Browser (http://www.aniseed.cnrs.fr/). (B) Alignment of the conserved 108bp CRE, labeling as described in Fig. 3. (C) Mutational analysis of ATTA co-motifs in RGS3 reporter constructs as indicated, labeling as described for previous figures (2–3 trials, N>80 for each sample). (D–E) Representative RGS3-1120-116:HL296:lacZ transgenic embryos co-electroporated with a Mesp-GFP construct. (D) Wildtype reporter construct drives robust reporter expression (red) in the TVCs (arrowheads). (E) ATTA mutations (A1/A2-mut, as diagrammed in C) abrogate TVC reporter expression (arrowheads). Scale bar = 20um.