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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Gastroenterology. 2012 Mar 27;143(1):110–121.e10. doi: 10.1053/j.gastro.2012.03.037

Figure 1. Two functional alleles of Ptgs2 are required for proper mucosal healing.

Figure 1

(A) Colonic sections from WT, (B) Ptgs2+/−, and (C) Ptgs2−/− mice 6 days post-injury were stained with antibodies against α-smooth muscle actin (α-SMA) (red), β-catenin (green), and bis-benzamide (nuclei, blue). Dotted yellow lines=muscularis propria, white dashed lines=adjacent crypts (AC), yellow arrowheads=epithelial barrier interruptions. Bars=100µm. (D) Graph of the percent loss of α-SMA underlying day 6 wound beds (gap length/wound bed length) that included data points (n≥6/genotype), median bar and significance by one-way ANOVA and post hoc Tukey's test: F2,18=10.33, **P<0.01. (E) Graph of the percentage of wound bed sections with incomplete epithelial coverage at day 6 post-injury. Wound bed sections were scored as either fully or incompletely covered by epithelial cells (β-catenin-positive). Fisher's exact test for the resulting data contingency table compared incomplete restitution in WT (2/14) to Ptgs2+/− (14/17) (P<0.001) and WT to Ptgs2−/− (7/9) (P<0.001).