Figure 2. Ptgs2 is induced in colonic wounds in a spatial and temporal manner.

(A) Graph of Ptgs2 mRNA expression (qRT-PCR) wounds at days 2, 4 and 6 post-injury (baseline=uninjured mucosa; n≥5 wounds per day). Data displayed as means ±SEM and significance by one-way ANOVA and post-hoc Tukey's test: F_2,18=5.33, *P<0.05. (B) Colonic sections from WT mice at days 2, 4 and 6 post-injury stained by in situ hybridization for Ptgs2. Solid black boxes=insets (right panels). Arrows indicated additional scattered Ptgs2 high-expressing cells outside of boxed area at day 2. The adjacent crypts (AC) and muscularis propria were outlined in red dashed lines. Bars=200µm; (50µm insets). (C) Graph of the number of Ptgs2 high-expressing cells per wound section. Data displayed as means ±SEM and significance by one-way ANOVA and post-hoc Tukey's test: F_2,9=11.6, *P<0.05. (D) Colonic sections from WT mice at days 2, 4 and 6 post-injury stained with anti-Ptgs2 antisera (green) and bis-benzamide (blue). White boxes=insets (right panels). Yellow dashed lines marked adjacent crypts (AC) and muscularis propria. Bars=100 µm (25µm inset). (E) Cartoon depicting the typical positional and temporal relationship of Ptgs2 high-expressing cells in the wound bed post-injury.