Fig. 5.

Neutrophils from inflamed FPR1^−/− mice respond to chemoattractants in a similar manner as neutrophils from inflamed C57BL/6 mice. Neutrophils were enriched to 95% from the bone marrow of both strains 22 days after starting a DSS cycle (acutely inflamed, as in Fig. 1) a Neutrophils were tested for migration to 10⁻⁸ M human recombinant C5a (protein fragment of complement component C5) and 10⁻⁸ M recombinant macrophage inflammatory protein 2 (MIP-2). Bars: Mean ± SD, n = 4 mice, one of two experiments (conducted using mice sacrificed on day 21). b MIP-2 levels in 24 h ex vivo colon culture supernatants from the same mice as in a (e.g., following one cycle of DSS). WT Wildtype (C57BL/6)