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. 2012 Jul;18(7):1338–1346. doi: 10.1261/rna.032268.112

FIGURE 3.

FIGURE 3.

Cytoplasmic requirements for processing of pri-miRNAs. Northern blot depicting WT MEFs or MEFs deficient in Drosha, DGCR8, Dicer, TRBP2, PACT, or AGO2 (Rnasen, Dgcr8, Dicer1, Tarbp, Prkra, or Eif2c2, respectively) mock-treated or infected with WT-rSINV or rSINV124 (MOI = 5 for Drosha and DGCR8-deficient cells; MOI = 1 for Dicer-, TRBP2-, PACT-, and AGO2-deficient cells; 16 h for all samples) and RNA was analyzed via small RNA Northern blot probed for miR-124 (top), miR-93 (middle), and U6 (bottom).