FIGURE 5.

Validation of newly identified non-mod(mdg4) trans-splicing events in the silkworm. (A) Schematics of two silkworm lines and their F₁ hybrids. (Red) mRNA and SNPs from JS line; (green) mRNA and SNPs from L10 line. (B) Exchanged SNPs from JS and L10 were observed in their F₁ hybrids to produce a chimeric RNA for six events, indicating that these events were through trans-splicing. Other events cannot be validated due to insufficient SNPs in the two trans-spliced genes. (C) Two trans-splicing events were further validated by using allele-specific RT-PCR and sequencing. cDNAs from JS, L10, and their mixtures were used as controls. Species-specific primers for TS5 and TS6 events were indicated in B. Sequences of products from each template were listed, and the SNPs were colored to indicate their origins.