Figure S5.

Cell Uptake of αS Species, Related to Figure 6

(A) Monomeric sample, (B) oligomeric A sample, (C) oligomeric B sample, and (D) fibrillar sample.

(i) Phase contrast images of rat primary neurons for each protein sample. Boxed areas show the cytoplasmic regions of cells utilized to record αS uptake. Cytoplasmic regions were specifically selected to ensure species uptake and not plasma membrane absorption was being measured.

(ii) Fluorescence signal of AF488, exciting at 488nm after the addition of each αS species sample.

(iii) Fluorescence signal of AF647, exciting the sample at 488nm to identify uptake of oligomeric and fibrillar species by FRET (monomers are unable to undergo FRET).

(iv) Magnified merged image of boxed area in corresponding images ii and iii.

(v) Profile of AF488 and AF647 fluorescence emission intensities along the red arrow in image iv. Simultaneous increase of both AF488 and AF648 signals, indicated by arrows, identify FRET signal within cell body. Images iii – v are absent for monomeric αS sample (A) because the monomeric species do not FRET.