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. 2011 Mar 24;69-540(6-7):1069–1084. doi: 10.1016/j.neuron.2011.02.018

Figure 5.

Figure 5

Both Rnd2 and Rnd3 Promote Neuronal Migration by Inhibiting RhoA Activity

(A) FRET analysis of RhoA activity in cortical cells in vivo, 1 day after Rnd2 or Rnd3 knockdown. Upper panels show the YFP signal from the FRET probe 1 day after electroporation; the RFP signal in insets marks electroporated cells; lower panels show FRET efficiency in the indicated area. Mean ± SEM; t test, n = 20 cells for each condition; ∗∗∗p < 0.001 compared to control; +p < 0.05 compared to Rnd2 shRNA.

(B) FRET analysis of RhoA activity in dissociated cortical cells in culture, 2 days after Rnd2 or Rnd3 knockdown. Mean ± SEM; t test, n = 15 cells for each condition; ∗∗∗p < 0.001 compared to control; +p < 0.05 compared to Rnd2 shRNA.

(C–E) Coelectroporation of RhoA shRNA fully rescued the radial migration defects of Rnd3-silenced neurons and partially rescued the radial migration defects of Rnd2-silenced neurons. Mean ± SEM; one-way ANOVA followed by a Fisher's PLSD post hoc test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bar represents 150 μm (C). See also Figures S5 and S6.