Figure 6.

Rnd3 and Not Rnd2 Promotes Migration by Depolymerizing F-Actin

(A) F-actin visualized with EGFP-UTRCH-ABD probe in dissociated cortical cells 2 days after coelectroporation of the probe and shRNAs. The RFP signals in insets mark electroporated cells. The graphs below the panels show the quantification of the green fluorescence of EGFP-UTRCH-ABD from a to b, as indicated in the panels above, by using ImageJ software. Knockdown of Rnd3 resulted in an accumulation of F-actin in the processes of electroporated cells, while F-actin accumulated in both cell body and processes of Rnd2 knocked-down cells.

(B and C) Coelectroporation of cofilin^S3A, a nonphosphorylatable form of cofilin that depolymerizes F-actin, fully rescued the migration defects of Rnd3-silenced neurons, but not those of Rnd2-silenced neurons. Mean ± SEM; one-way ANOVA followed by a Fisher's PLSD post hoc test; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Scale bars represent 10 μm (A) and 150 μm (B, C).