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. 2011 Mar 24;69-540(6-7):1069–1084. doi: 10.1016/j.neuron.2011.02.018

Figure 8.

Figure 8

Rnd3 Function Diverges from that of Rnd2 Because of Its Localization to the Plasma Membrane

(A) Subcellular localization of Flag-Rnd2, Flag-Rnd3, and a version of Rnd2 containing the C-terminal end of Rnd3 (Flag-Rnd2Rnd3Cter) transfected in HEK293 cells. Quantification of fluorescence below the panels is as described in Figure 7D. Flag-Rnd3 and Flag-Rnd2Rnd3Cter, but not Flag-Rnd2, colocalize with the plasma membrane.

(B) Rnd2Rnd3Cter was as active as wild-type Rnd3∗ at rescuing the migration defects of Rnd3-silenced neurons, while wild-type Rnd2 had no activity in this assay. Mean ± SEM; one-way ANOVA followed by a Fisher's PLSD post hoc test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bars represent 10 μm (A) and 150 μm (B). See also Figure S8.