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. 2012 Jul 1;367-540(1-7):55–65. doi: 10.1016/j.ydbio.2012.04.025

Fig. 3.

Fig. 3

Otx2 and Gbx2 mutually repress each other in the PPR. (A) and (B) Injection of Otx2 mRNA into the D1 blastomere of 8-cell stage embryos (A) shifts En-1 posteriorly on the injected side (50%. n=28; FDX: turquoise), while injection into the A3 blastomere of a 32-cell stage embryo has little effect ((B); 5% affected, n=36). (C) and (D) Injection of Gbx2 mRNA into the D1 blastomere at 8-cell stage (C) shifts En-1 anteriorly on the injected side (68%; n=64; FDX: turquoise), while injection into the A3 blastomere at 32-cell stage has no effect ((D); 0% affected, n=31). Dorsal view, anterior to the top. (E) and (F) Injection of Otx2 (E; 68% affected, n=31) or Otx2-EnR ((F); 77% affected, n=17) into A3 at the 32-cell stage inhibits Gbx2 in the PPR. Compare bracket in the injected (FDX: turquoise) and uninjected side. (G) and (H) Injection of Gbx2 into A3 at the 32-cell stage leads to Otx2 repression (68%; n=26); compare brackets (G) on the injected ((H): FDX, turquoise) and uninjected side. (I) and (J) Co-injection of splice and translation blocking Gbx2 morpholinos into A3 at 32-cell stage leads to Otx2 expansion (73%; n=33); compare brackets (I) on the injected ((J) FDX, turquoise) and uninjected side. (K) and (L) Injection of Gbx2-EnR into A3 at the 32-cell stage leads to a loss of Otx2 in 62% of embryos (n=52); compare brackets (K) on the injected ((L) FDX, turquoise) and uninjected side. (E)–(L) Frontal view, dorsal to the top.