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. 2012 Jul 1;367-540(1-7):55–65. doi: 10.1016/j.ydbio.2012.04.025

Fig. 6.

Fig. 6

Activation of Otx2 target genes is required at early and late stages of lens placode formation. (A), (B) Otx2-EnR inhibits Pax6 in the lens at stage 17 (50%, n=26). This is rescued by co-injection of Otx2 ((B); inhibition reduced to 15%, n=20). (C)–(F) Activation of Otx2-EnR-GR at stage 10 reduces lens-specific Pax6 at stage 18 ((D) 52%, n=25); without DEX Pax6 expression is normal ((C) 0% affected, n=23). FoxE3 at stage 25 is normal without DEX ((E) 0% affected, n=30), while addition of DEX at stage 18 leads to reduction ((F) 64%, n=22). (G) Otx2-E1A activates Otx2 targets but does not affect lens Pax6 (0% affected, n=25). All embryos were injected into the A3 blastomere at the 32-cell stage and are shown in frontal view with dorsal to the top. High magnifications of the lens region are shown below each panel; dotted lines demarcate placodal Pax6. Turquoise staining reveals the lineage tracer FDX.