Fig. 2.

Global analysis of chaperone effects on the prevention of aggregate formation. (A) Typical examples. SDS-gels of four aggregation-prone E. coli cytosolic proteins (asd, hemB, yedS, and yajB) in the absence and the presence of the chaperones are shown. The numbers below the electrophoretic pattern indicate the solubility values, calculated by the ratio of the amount of translation products in the Sup (S) and Total (T) fractions. (B) Histograms of solubilities in the presence of three E. coli chaperones. The aggregation-prevention effect is represented as Δsolubility, defined by subtracting the solubilities in the absence of chaperones from those in the presence of each chaperone (see Fig. S2B for raw data).