Fig. 1.

Cse4 is methylated at arginine 37. (A) Collision-induced dissociation analysis and full annotation of the parent ion (Cse4 27-LAGDQQSINDR_me-37) with m/z = 615.81. Score and expected values as calculated by Mascot are reported, and fragments detected in the experiment are indicated in color (blue for b ions and red for y ions). (B) Specificity of antibodies generated against Cse4-R37me1 and R37me2a. The indicated amount of Cse4 peptide carrying R37me2a (Left) or R37me1 (Right) was spotted on nitrocellulose membrane. Antibodies were preincubated with the competing peptides indicated to the right of the blots. (C) Histone extracts from WT (AEY2781) and cse4-R37A (AEY5040) cells were probed with α-Cse4-R37me1 and R37me2a antibodies, as well as with α-HA to detect 3xHA-Cse4 as a loading control.