Fig. 3.

Mutation of Cse4-R37 caused synthetic lethality and growth defects when combined with mutations in genes encoding Ctf19 complex components. (A) Tetrad dissection of genetic crosses of cse4-R37A with isogenic W303 strains carrying ctf19Δ (Left) or mcm21Δ (Right). The four spores from individual asci are aligned in vertical rows. Double mutants are marked with circles. (B) cse4-R37A caused a synthetic growth defect in the absence of the Ctf19 complex component Iml3/Mcm19. Serial dilutions of four segregants of a tetrad from a cross between cse4-R37A and iml3Δ were spotted on complete medium and grown at the indicated temperatures for 3 d. The top two rows show the parental strains. (C) cse4-R37A caused a synthetic growth defect with chl4Δ. Representation as in B.