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In vivo λ recombination assays. (A) Schematic of λ recombination assay. (B) Recombination frequency (%). (C) Hotspot activity (ratio of turbid/clear plaques). Averages with SDs from at least three assays. Recombination frequency of χ0 and χ+ λ for wild-type strain (chromosomal recC+ gene) was 0.63 ± 0.16 and 2.8 ± 0.5, and hotspot activity was 1.8 ± 0.2 and 7.2 ± 0.6, respectively. *P < 0.005 in t test.
