Fig. 1.

Steady-state DCs induce priming of functional CTLs instead of tolerance after CD4 depletion. DIETER mice were injected i.v. with 0.5 mg of GK1.5 (closed symbols) or isotype control (open symbols) on day −1. On day 0, antigen presentation was induced by injection of 2 mg of tamoxifen i.p. (A) On day 8, expansion of GP33-41 specific CTLs was detected in the blood by staining with MHC class I tetramers. (B) On day 8, mice were challenged with 200 pfu of LCMV-WE i.v., and virus titers in the spleen were determined on day 13. n = 7 per group. Results are representative of four independent experiments.