Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Type 2 (relaxed-specificity) mutants stimulate χ-dependent joint molecule formation, whereas type 1 (lost-recognition) mutants do not. Coupled RecABCD reactions (illustrated) were conducted with enzymes for times indicated. Mobilities of full-length ssDNA, χ-specific ssDNA fragments, and joint molecules are indicated. The final yields (± SD) of χ-dependent joint molecules are from at least three independent experiments; ND signifies not detectable (<0.2%).
