Fig. 5.

Adenovirus-mediated expression of APPL1 enhances SNARE protein expression and insulin secretion in the islets of APPL1 KO mice. (A) Islets isolated from 20-wk-old male APPL1 KO mice on STC were infected with recombinant adenovirus expressing APPL1 or luciferase (Luci) for 36 h, followed by Western blot analysis to detect the expression levels of the three SNARE proteins. Bar chart on the Right is the densitometric analysis for the relative abundance of the three SNARE proteins in the infected islets. (B) Static insulin secretion in the infected islets measured after stimulation with glucose or KCl for 30 min. (C) First phase (6–18 min) and second phase (18–42 min) of GSIS of the infected islets using the perifusion system. (D) AUC for the first and second phase of insulin secretion during the perifusion experiment as in C. *P < 0.05 (n = 8). NS, not significant.