Fig. 3.

Surgical removal of the parametrial fat pads decreases high-fat-diet–induced TIMP1 and other adipokines in the epidermis and in the serum. Female SKH-1 mice (10 mice per group) were given either a 40%-kcal high-fat diet or a 60%-kcal very-high-fat diet for 2 wk. Half of mice had their parametrial fat pads removed and the other half of mice was a sham-operated control. Two weeks after the surgery, the mice were killed, and the epidermis and serum were collected, combined separately, and subjected to antibody array for adipokines. Representative arrays are presented. (A) Epidermis from sham-operated control mice and epidermis from lipectomized mice fed a 40%-kcal high-fat diet. (B) Serum from sham-operated control mice and serum from lipectomized mice fed a 40%-kcal high-fat diet. (C) Epidermis from sham-operated control mice and epidermis from lipectomized mice fed a 60%-kcal very-high-fat diet. (D) Serum from sham-operated control mice and serum from lipectomized mice fed a 60%-kcal very-high-fat diet.