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The genetic modification of chicken primordial germ cells using DNA transposons. 1. PGCs are isolated from chicken embryonic blood and propagated in vitro. 2. DNA transposons are introduced into PGCs and stably integrate into the genome. GOI, gene of interest. 3. The modified PGCs are injected into host embryos and raised to sexual maturity. 4. The genetically modified PGCs form functional gametes and produce offspring containing the DNA transposon.
