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. 2012 May 14;109(23):E1466–E1472. doi: 10.1073/pnas.1118715109

Fig. 2.

Fig. 2.

Stable genetic modification of primordial germ cells using piggyBac and Tol2 vectors. Chicken PGCs were transfected with transposon constructs with (+) or without (−) transposase and visualized a minimum of 2 wk after transfection for GFP expression. (A) Minus transposase control; (B) PB-CGIP vector; (C) Tol2-CGIP vector. (Scale bar, 50 μm.) (D) Comparison of stable transfection rates of piggyBac and Tol2 transposons in PGCs. Data are corrected for transfection efficiency and represent a minimum of four independent experiments, using two lines of PGCs. Error bars, SEM. **P < 0.01.