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. 2012 Apr 10;13:13. doi: 10.1186/1471-2199-13-13

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Copyright ©2012 Zylicz-Stachula et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of allosteric cofactors on TspDTI REase activity. 1 μg (= 5.58-pmol restriction sites) bacteriophage λ DNA was digested with 2.79 pmol TspDTI (0.5:1 M ratio of enzyme to recognition sites) for 30 min at 70°C and electrophoresed on 1.2% agarose/TBE gel. Lane M1, 1 kb ladder (selected bands marked); lane M2, 100 bp ladder (selected bands marked); lane K, undigested λ DNA; lane 1, (+ TspDTI, no allosteric cofactor; lane 2, (+ TspDTI, + 50 μM SIN); lane 3, (+ TspDTI, + 50 μM SAM); lane 4, (+ TspDT, + 50 μM SAH); lane 5, (+ TspDTI, + 50 μM ATP).