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Annealing study of non-telomeric GQ-forming sequences G4TERT, Bcl-2 and cKit1 in the presence of NMM in 5K buffer. Samples of 4–6 µM DNA were annealed at 90°C for 10 min without (solid lines) or with (dotted lines) 2 equivalents of NMM, cooled to 30°C slowly and stored at 30°C for >12 h before collecting CD.
