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. 2012 Feb 23;40(12):5432–5447. doi: 10.1093/nar/gks152

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — FRET melting of F21D in the presence of porphyrins and competitors. (A) Increase in the stabilization temperature, ΔT_1/2, of 0.2 µM F21D as a function of porphyrin concentration. (B) Stabilization of F21D by NMM or NMP in the presence of duplex DNA competitors, ds26 or CT or (C) parallel stranded GQ competitors [dTG₅T]₄, G5, and [dTG₈T]₄, G8. Concentrations of porphyrins and F21D were fixed at 1.6 and 0.2 µM, respectively. Amount of competitor is indicated in the figure legend. All experiments were performed in 5K buffer.