Table 2.

Stabilization temperature, ΔT_1/2 (°C), for porphyrins under study in FRET melting assays

DNA/porphyrin	NMM	NMP	MIX	PIX	TMPyP4	ZnTMPyP4
F21D, 5K	14 ± 2	8.5 ± 0.6	0.2 ± 0.3	0.2 ± 0.3	34 ± 2	23 ± 1
FGD, 5K	12.5 ± 0.3	9.8 ± 0.3	0.8 ± 0.3	0.2 ± 0.3	31 ± 2	22.1 ± 0.9
F21D, 50Na	0.4 ± 0.5	0.3 ± 0.3	–	–	30.6 ± 0.3	16.4 ± 0.7

The melting temperatures were recorded at 0.2 µM DNA and 1 µM porphyrins.