Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 1;40(12):5775–5786. doi: 10.1093/nar/gks168

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by Oxford University Press 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Orthogonal theophylline-sensing pT181 fusions based on the strand-exchange design. (A) The region that determines the target-specificity of pT181 ncRNA is in the cyan box (4), and allosteric region is in the black box. The sequences of theophylline-sensing WT pT181 and MT pT181 fusions are shown. (B) Experimental setup to test theophylline-induced target specificity of theo–SE–pT181WT and theo–SE–pT181MT. All four possible combinations between aptamer–ncRNA fusions and reporters were assayed with and without theophylline. (C) Fluorescence assay data with red bars showing (−) theophylline and blue bars showing (+) 2.5 mM theophylline.