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. 2012 Mar 1;40(12):5775–5786. doi: 10.1093/nar/gks168

Figure 5.

Figure 5.

Designed MS2 coat protein-sensing pT181 ncRNA fusions. (A) Sequences of the MS2–SE–pT181 aptamer–ncRNA fusion screened from five designed mutants (Supplementary Figure S10). (B) Fluorescence assay of MS2–SE–pT181 ncRNA fusions with intracellular MS2 coat protein induced by IPTG. The repression percentage between 500 µM and 1 nM IPTG is 87.5% compared to 89.1% between positive and negative controls. The inset shows the cytometry histogram of three IPTG concentrations. (C) A NOR logic engineered from theo–SE–pT181WT and MS2–SE–pT181WT aptamer–ncRNA fusions. The two aptamer–ncRNA fusions control the same target and integrate ligand signals in a way that presence of any ligand represses the target gene expression. A theoretical NOR truth table is shown in the plot.