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. 2012 Mar 2;40(12):5477–5486. doi: 10.1093/nar/gks180

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Published by Oxford University Press 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Immunological detection of the DNA-derived radical adduct from the reaction of cellular DNA obtained from RAW cells treated with CuCl₂ and H₂O₂ in the presence of DMPO at 37°C for 1 h. (A) Shows the time course of cell viability. (B) All components of the reaction must be present for adduct formation. (C) The cytosolic and the nuclear fractions of the RAW cells were separated and the total DMPO adducts (both protein and DNA) determined in both fractions. (D) DNA was extracted from both the cytosolic and the nuclear fractions, concentration determined and DNA–DMPO adduct determined.