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. 2012 Mar 2;40(12):5477–5486. doi: 10.1093/nar/gks180

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Published by Oxford University Press 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Confocal laser scanning microscopy of DNA-derived radical adducts in RAW 264.7 cells. Adherent RAW 264.7 cells in glass-bottomed 32 mm² dishes were treated with CuCl₂/H₂O₂ in the presence of 25 mM DMPO for 1 h. Cells were fixed with paraformaldehyde and stained with the anti-DMPO antibody, which was detected with an Alexa 488 conjugated secondary antibody. (A) Cells were imaged and anti-DMPO immunoreactivity (green stain) could be seen primarily in the nucleus (DAPI, blue stain), as evidenced by the co-localization (overlay image). (B) There was no DMPO immunoreactivity in cells that were not treated with the spin trap.

Figure 3. — Confocal laser scanning microscopy of DNA-derived radical adducts in RAW 264.7 cells. Adherent RAW 264.7 cells in glass-bottomed 32 mm² dishes were treated with CuCl₂/H₂O₂ in the presence of 25 mM DMPO for 1 h. Cells were fixed with paraformaldehyde and stained with the anti-DMPO antibody, which was detected with an Alexa 488 conjugated secondary antibody. (A) Cells were imaged and anti-DMPO immunoreactivity (green stain) could be seen primarily in the nucleus (DAPI, blue stain), as evidenced by the co-localization (overlay image). (B) There was no DMPO immunoreactivity in cells that were not treated with the spin trap.