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. 2012 Feb 29;40(12):5615–5624. doi: 10.1093/nar/gks195

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Development of male germ-line in smg7-6 and smg7-4 mutants. (A–D) Anthers of different smg7 mutants after Alexander staining. Viable pollen stain red, no pollen is present in smg7-1 anthers. Inset in (D) shows four joint pollen grains in smg7-4 mutants. (E–J) End of second meiotic division in smg7-1 and smg7-6 mutants. Meiotic chromosomes are stained with DAPI: (E) regular anaphase II, (F, I) irregular anaphase II, (G) slowly decondensing chromosomes after anaphase II, (H) regular telophase II, (J) a polyad consisting of five separated cells with irregular nuclei. (K, L) Microspores typical for smg7-4 mutants remain attached after meiotic division. Nuclei are stained with DAPI (K), cell walls detected due to their auto-fluorescence using FITC filters (L). (M, N) Fields of microspores in wild-type and smg7-6 mutants.