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. 2012 Mar 1;40(12):5357–5367. doi: 10.1093/nar/gks198

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Enhanced expression of TTF-I is inhibitory to rRNA synthesis and processing. (A) Conditional expression of FLAG-TTF-I in tet-off regulated cell clones B6 and F9, and localization of endogenous fibrillarin (Fib) before and after induction of the UBF transgene by withdrawal of doxycyclin (Dox+, Dox–). (B) B6, F9 cell clones and the NIH3T3 cell line were maintained in the presence or absence of doxycyclin were subjected to a 3-h [³H]-uridine pulse-labelling and incorporation into rRNA determined in parallel with the immunoblot analysis of total TTF-I (anti-TTF#3), FLAG-TTF-I and fibrillarin protein levels. (C) Quantitation of three independent pulse-label analyses as in B. 45S rRNA synthesis is shown in the left-hand panel and the ratios of 28S:45S and 18S:45S rRNAs before and after doxycycline removal are shown in the middle- and right-hand panels for cell clones B6 and F9 and NIH3T3 cells.