Figure 8.
Co-localization of ORC1N1–238–GFP and Sir2 and immunolocalization of ORC1 and HP1 in 3D7 and Sir2 KO parasite lines. Glass slides containing the parasite smears from ORC1N1–238–GFP expressing parasites or 3D7 wild-type or Sir2 KO parasite lines were treated for immunolocalization using respective antibodies as indicated in the panels (A) and (B). (A) Co-localization of GFP and Sir2 in ORC1N1–238–GFP parasites during early stages of development as indicated on the left. ‘R’ indicates ring stage and ‘ET’ indicates early trophozoite stage parasites. DAPI shows the nuclei. [B(i)] Both ORC1 and HP1 show nuclear punctate staining in 3D7 wild-type parasites during the early stages of development. [B(ii)] ORC1 shows more diffused and distributed pattern while HP1 shows punctate staining in the Sir2 KO parasites. ‘R’ indicates ring stage and ‘S’ indicates schizont stage parasites.