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. 2012 Feb 8;40(12):5679–5693. doi: 10.1093/nar/gks223

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Air2p N- and C-termini and ZnK4 and ZnK5 domains are important for TRAMP functions and integrity. (A) Overview of mutant forms of AIR2 used in this study: ZnK, zinc knuckle. The aminoacid substitutions of individual point mutants are designated in brackets. (B) Growth test analysis of WT and mutant forms of Air2p episomally expressed in air1Δair2Δ strain, serially diluted on SD-Leu media and incubated for 3 days at temperatures indicated. (C) Northern blot analysis of total RNA isolated from AIR2 WT and mutant yeast strains grown at 25°C, with specific probes for RNAs indicated. The number below each lane represents percentage of unprocessed snoRNA precursor. (D) Western blot analysis of the composition of purified TRAMPs containing WT and mutant Air2p variants. TRAMP subunits were detected with antibodies indicated: HSW, high-salt wash (1M KCl).