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. 2012 Feb 9;40(12):5415–5431. doi: 10.1093/nar/gks225

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Mod67.2 protein facilitates chromosome association of Su(Hw). (A) Shown are the polytene chromosomes from third instar larval salivary glands of su(Hw)^WT CP190^WT mod(mdg4)^WT, su(Hw)^WT CP190^H4⁻^1/P11 mod(mdg4)^WT and su(Hw)^WT CP190^WT mod(mdg4)^u1 backgrounds stained with α-Su(Hw) antibody (green) and DAPI (blue/white). For each genotype, the tip of the X chromosome is enlarged, with cytological positions indicated on the right. (B) Shown are ChIP-qPCR analyses of Su(Hw) binding in ovaries of wild-type (top), mod(mdg4)^u1 (middle) or CP190^H4⁻^1/P11 (bottom) mutants. SBSs that were analyzed fall into two subclasses, SBS-CM (blue) and SBS-O (gray). Error bars indicate standard deviation of two to four independent biological replicates. *P ≤ 0.01 (Student’s t-test).