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Effects of 2 mg/kg rapamycin on cortical phosphorylated S6 levels and GFAP expression after behavioral testing. (A) There is increased expression of pS6 levels in the cortex of both the postnatal and combined groups, indicating that 2 mg/kg of rapamycin was not sufficient to maintain the mTORC1 inhibition observed at P21 after 0.1 mg/kg (Fig. 2). (B) The increased pS6 expression is accompanied by cortical astrogliosis. (C) Merge of A and B.
