Figure 4.

Cohesin complexes in the absence of RAD21L and REC8. Double immunofluorescence of SYCP3 and either RAD21, SMC3, SMC1β, or STAG3. In wild-type leptotene spermatocytes, the cohesins RAD21, SMC3, SMC1β, and STAG3 colocalize with SYCP3 along the AEs of the chromosomes. In Rad21l and Rec8 single mutant leptotene spermatocytes, RAD21, SMC3, SMC1β, and STAG3 colocalize also with SYCP3 along the AEs/LEs of the chromosomes. In spermatocytes from dKO-kls, arrested at leptonema, SMC1β and STAG3 are not detected by immunofluorescence, whereas immunolabeling for RAD21 and SMC3 renders robust and very faint fluorescence signals, respectively. Bar, 100 µm.