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. 2012 Jun 25;197(7):897–905. doi: 10.1083/jcb.201203062

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Copyright © 2012 by the Rockefeller University Press

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Figure 2. — Taxon-specific sperm binding. (A) Sperm-binding assay in which 10–20 eggs in cumulus were added to 10⁶ ml⁻¹ sperm in HTF (supplemented with 0.5% BSA) and incubated for 4 h at 37°C. After serial washing with control eggs or two-cell embryos, eggs were fixed to determine the number of bound sperm. (B) Capacitated human sperm binding to noninseminated human oocytes, normal mouse, and Zp3^EGFP transgenic eggs. (top) Confocal (blue channel) z projections were obtained after staining with Hoechst. (bottom) DIC and confocal (green channel) images were merged to detect zonae pellucidae containing Zp3^EGFP. (C) Same as B but with capacitated mouse sperm incubated for 1 h.