Figure 6.

The IGR-CaP1 model expresses a set of human bone metastasis genes. (A) The relative expression of specific human bone metastasis genes in bone lesions was determined using real-time quantitative RT-PCR. Gene expression was detected in IGR-CaP1-injected tibias (n = 7; gray bars) and IGR-CaP1-xenografted tumors (n = 4; black bars) but not in PBS-injected control tibias. The results were normalized using the human GAPDH gene and were expressed relative to the universal reference RNA. (B) Immunohistochemical staining showing expression of intracellular MMP-9 and nuclear Runx2 in either intraprostatic tumors (upper figures) or decalcified bone sections (lower figures). Magnification, x100; insert, x400.