Table 2.
EGFcyt Coding Region of Peptides | Peptide No. | Peptide Sequence (N′- to C′-terminus) |
Exon 22 | P1 | TQKLLSKNPKNP |
P2 | KNPYEESSRDVR | |
P3 | DVRSRRPADTED | |
P4 | TEDGMSSCPQP | |
Exon 23 | P1 | PQPWFVVIKE |
P2 | VIKEHQDLKN | |
P3 | DLKNGGQPVA | |
P4 | QPVAGEDGQAAD | |
Scrambled peptide | P5 | QPVIHDGVAAE |
Peptides (10–12 mer) were designed to cover the complete exons 22 and 23 and have a three amino acid overlap at the N-terminus with each consecutive C-terminal peptide. All peptides were FITC conjugated at the N-terminus to monitor cellular uptake.