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. 2012 May;14(5):440–450. doi: 10.1593/neo.12352

Figure 2.

Figure 2

BART binds to Rac1 at the leading edges of migrating cells. (A) Immunoprecipitation of endogenous BART or Rac1 from S2-013 cells. Anti-BART or anti-Rac1 immunoprecipitates were examined by Western blot analysis using anti-BART and anti-Rac1 antibodies. Rabbit IgG and mouse IgG monoclonal antibodies were used as isotype controls for anti-BART and anti-Rac1 antibodies, respectively. Data are representative of three independent experiments. (B) Immunocytochemical staining of S2-013 cells using anti-BART (green) and anti-Rac1 (red) antibodies. Arrow indicates colocalized BART and Rac1 in lamellipodial-like protrusions; blue, DAPI staining. Bar, 10 µm. (C) Confluent S2-013 cells were wounded. After 4 hours, the cells were immunostained using anti-BART (green) and anti-Rac1 (red) antibodies. Arrows indicate colocalized BART and Rac1 at the leading edge; blue, DAPI staining. Bar, 10 µm.