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. 2012 Apr 10;5:20. doi: 10.1186/1754-6834-5-20

Table 2.

Domain-level primers used to amplify short-subunit rDNA genes from DNA extracts of biomass compost

Genes Primer sequences & reference Amplicon size(bp)
Archaea 16 s rDNA F: TTCCGGTTGATCCYGCCRG
R: YCCGGCGTTGAMTCCAATT
Ref: [22]
937

Bacteria 16 s rDNA F: TCCTACGGGAGGCAGCAGT
R: GGACTACCAGGGTATCTAATCCTGTT
Ref: [25]
466

Fungi 5.8 s and ITS2 rDNA F: GCATCGATGAAGAACGCAGC
R: TCCTCCGCTTATTGATATGC
Ref: [22,26]
400