Figure 6.
NO production and inhibition of in-stent restenosis with immobilized AdiNOS vectors. A, Stainless steel mesh-immobilized AdiNOS results in more efficient NO production than MOI equivalents of free adenovirus. Relative NO production (per Griess assay) after transduction of A10 cells with unmodified AdiNOS (MOI range 1 to 500) or with 1.6×108 AdiNOS particles (corresponding to MOI of 60) tethered via PABT/PEI(PDT)/HL complex formation. B and C, Significant inhibition of in-stent restenosis with stent delivery of immobilized AdiNOS. Representative photomicrographs of arterial sections treated with control BMS (B) and AdiNOS-tethered stents (C). Verhoeffvan Gieson elastic stain, original magnification ×200.