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. Author manuscript; available in PMC: 2012 Nov 11.
Published in final edited form as: Cell. 2011 Oct 27;147(4):840–852. doi: 10.1016/j.cell.2011.09.045

Figure 5. Increase in SREBP-1 processing and target gene expression in Ctα knockout mouse livers.

Figure 5

A. Immunoblotting of extracts from mice with a liver-specific knockout of Ctα (Jacobs et al., 2004) limiting the CDP-choline pathway shows increased SREBP-1a/c processing (Jacobs et al., 2004). Fl is full-length SREBP-1a/c precursor; I, intermediate form; M, mature, transcriptionally active form. B. Analysis of the immunoblot by densitometry shows increases in proteolytic products of SREBP-1 in livers from Ctα knockout mice. C. Analysis of gene expression by qRT-PCR from Ctα knockout mice show increased expression of Scd1, an SREBP-1 target, as well as Mat1a, ortholog of sams-1. Bars represent individual mice, error bars show standard deviation; statistical relevance (p value<0.01) shown by (**). Analysis of gene expression by qRT-PCR from the liver-specific Ctα knockout mice (Jacobs et al., 2004) show no statistically significant changes in levels of mature, processed SREBP-2 (D) or target gene expression (E). Bars represent individual mice, error bars show standard deviation; statistical relevance (p value<0.01) shown by (**).