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. Author manuscript; available in PMC: 2012 Jun 27.
Published in final edited form as: Curr Stem Cell Res Ther. 2009 Jan;4(1):44–49. doi: 10.2174/157488809787169138

Fig. 3. Time course of tumor infiltration by neuralized embryonic stem cells on an organotypic brain slice.

Fig. 3

These stereoscopic epifluorescent images show the progression of tumor mass infiltration by nESCs over the course of a several weeks in an organotypic brain slice culture. GFP-expressing nESCs and RFP-expressing human glioma cells were introduced on the surface of an organotypic rat brain slice as described in Fig. 2. (A–C) Starting at 2 weeks post-implantation, nESCs are found at the tumor mass. At 3 weeks (D–F) and 4 weeks (G–I) post-implantation, the tumor mass becomes infiltrated progressively by nESCs. At 6 weeks (J–L), the tumor mass becomes encapsulated completely by the stem cells, suggesting contiguity between nESCs and glioma cells. Scale bar = 1 mm; Scale bar applies to all panels.